Table 2.

Gradient Delay Volume Measured by Each Approach under Different Experimental Conditions on Instrument A

Gradient Length (min)	Flow Rate (mL/min)	Stationary Phase Batch	Column Dimensions	Measured by MYG		Measured by Modified Conventional Procedure
				Mixing Volume (mL)	Total Delay Volume (mL)	Mixing Volume (mL)	Total Delay Volume (mL)
5	0.4	2	2.1x100 mm	0.158	0.364	0.066	0.341
10	0.4	2	2.1x100 mm	0.095	0.356	0.070	0.360
10	0.1	2	2.1x100 mm	0.161	0.36	0.073	0.295
10	0.2	2	2.1x100 mm	0.105	0.355	0.086	0.322
10	0.4	1	2.1x150 mm	0.045	0.338
10	0.4	1	2.1x100 mm	0.087	0.359
10	0.4	1	2.1x50 mm	0.08	0.373
10	0.4	1	3.0x100 mm	0.068	0.344
10	0.4	3	2.1x100 mm	0.055	0.331
10	0.4	4	2.1x100 mm	0.092	0.366
10	0.4	5	2.1x100 mm	0.095	0.368
10	0.4	6	2.1x100 mm	0.057	0.345
Average:				0.092±0.037	0.355±0.013	0.074±0.009	0.329±0.028