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. Author manuscript; available in PMC: 2015 Nov 20.
Published in final edited form as: Cell Rep. 2014 Nov 6;9(4):1273–1280. doi: 10.1016/j.celrep.2014.10.014

Figure 1. TALEN-mediated inactivation of the gene for human Rap1.

Figure 1

(A) Schematic of human Rap1, the TERF2IP locus, the targeting construct, and the resulting knockout allele. F1, R1, and R2neo: PCR primers for genotyping. Arrows in bold: TALEN binding and cut sites.

(B) PCR genotyping of the TERF2IP gene and western blotting for Rap1 in the indicated clones. +, WT allele; Δ, targeted allele; mut, mutation resulting in loss of Rap1.

(C) Southern blot of EcoRI (RI)- or BglII/EcoRV (RV)-digested genomic DNA from targeted HT1080 clones. Probe shown in (A).

(D) Telomeric ChIP of WT and targeted HT1080 clones. Duplicate dot-blots were probed for telomeric or Alu repeats.

(E) Average percentage of telomeric DNA recovered in ChIPs with the indicated antibodies (two independent experiments). Error bars: SEMs.

(F) Schematic illustrating NHEJ and HDR after TALENs cutting.

(G) Table indicating number of Rap1 knockout clones acquired and the genetic alterations in TERF2IP.

See also Figure S1.