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. Author manuscript; available in PMC: 2015 Nov 24.
Published in final edited form as: Dev Cell. 2014 Nov 13;31(4):474–486. doi: 10.1016/j.devcel.2014.10.004

Figure 5. Chinmo is required autonomously in adult CySC lineage cells to prevent their transformation into female soma.

Figure 5

(A–G) Immunofluorescence detection of FasIII (green at cell periphery) and the germ cell marker Vasa (red) to visualize the morphology of adult testes after expression of chinmo-RNAi in different cell types. Before RNAi induction in the CySC lineage (A), testes appear normal. After RNAi induction in the CySC lineage, testes resemble chinmoST testes: somatic cells initially form aggregates (97% of testes, n = 36/37) (B–C, arrowheads) and then follicle-like cells (76% of testes, n = 68/90) (D). RNAi induction in the CySC lineage with a different Gal4 driver also phenocopies chinmoST testes (E). RNAi induction in germ cells (F) or in hub cells (G) does not phenocopy chinmoST. Hubs marked by asterisk. Scale bars = 20 μm. (H) Composite bar graph showing the percentage of testes with normal, mild, or severe phenotypes after expression of chinmo-RNAi with different somatic drivers. Testes remain morphologically wild type after chinmo-RNAi expression only in cyst cells (eyaA3-Gal4 at 25 °C), but after expression in both CySCs and cyst cells (eyaA3-Gal4 at 29 °C, c587-Gal4, or tj-Gal4), testes display a range of chinmoST phenotypes, as defined in Methods. See also Figure S2, Table S3.