Table 1.
Overexpressing DsxM in the CySC lineage rescues Chinmo knock down phenotype
| Use c587-Gal4 to express: [1] | % testes with FasIII+ somatic aggregates [2] | |||||
|---|---|---|---|---|---|---|
| 0 d | 4 d | 7 d | 9 d | 11 d | 14d | |
| UAS-chinmoRNAi-1 | 0 (n=31) | 6.3 (n=64) | 73.8 (n=103) | 80.5 (n=133) | 97.1 (n=35) | 100 (n=59) |
| UAS-chinmoRNAi-1, UAS-DsxM | 0 (n=25) | 5.3 (n=76) | 36.6 (n=153) | 55.9 (n=145) | 39.4 (n=33) | 86.3 (n=124) |
| UAS-DsxM | 0 (n=18) | N/A | 0 (n=24) | 5.3 (n=19) | 0 (n=21) | N/A |
[1]
UAS-chinmoRNAi-1 = chinmoHM04048
[2]
All flies were raised at 18 °C, which suppresses RNAi induction. After eclosion, adult flies were shifted to 29 °C for the indicated amount of time to induce RNAi
[3]
For DsxM rescue, p<0.0001 at 7, 9, 11days, P<0.01 at 14 days