Figure 6. Subcellular distribution of Iav, hTRPV1, and hTRPV4 in Drosophila larval motor neurons.
(A-C) Confocal images of MN cell bodies in ventral nerve cord dissected from larvae expressing Iav and KDEL-GFP in motor neurons stained with αIAV (A, magenta), αGFP (B, green), and merge (C). Scale bar shown in (A) also applies to (B-C).
(D-F) Same as (A-C) but with larvae overexpressing hTRPV1 instead of Iav. An antibody against hTRPV1 was used. Scale bar shown in (D) also applies to (E-F). Arrows indicate colocalization between hTRPV1 and KDEL-GFP in the nuclear envelope and other regions of the ER.
(G-I) Same as (A-C) but with larvae overexpressing hTRPV4 instead of Iav. An antibody against hTRPV4 was used. Scale bar shown in (G) also applies to (H-I). The arrowheads point to “tubular” structures that are decorated by hTRPV4 in motor neuron cell bodies.