Table 2. Groups and study design for experiments with human dermal microvascular endothelial cells (EC) and bone-marrow derived stem cells (BMSCs) co-culture in bioactive glass (BG) scaffolds.

Scaffoldtype	Cells inscaffold	Groupnames	Microscopy	acLDL uptakeassay	Tubeformation assay	FACSanalysis	TimePoints
–	–	I	(All)	2	2	6	Week 1, Week 2
BG	–	II	(All)	2	2	6	Week 1, Week 2
BG with 1% Cu2+	–	III	(All)	2	2	6	Week 1, Week 2
BG	BMSCs	IV	(All)	2	2	6	Week 1, Week 2
BG with 1% Cu2+	BMSCs	V	(All)	2	2	6	Week 1, Week 2

The ECs were always seeded on bottom, while the scaffolds with or without BMSCs are in the cell culture insert. For this experiment, we used a cocktail media consisting of one part of basal culture media and one part of endothelial growth media (EGM) without added VEGF component. For each type, 10 wells were used as per the distribution shown below. The collected media were used to estimate VEGF by ELISA and Cu²⁺ content.