Table 2.
Rose Bengal IC50 (μM) inhibition of SecA channel activity in oocytes
The IC50 of channel activities for Rose Bengal was determined with liposomes reconstituted with SecAs (120 ng) of E. coli (EcSecA), Bacillus subtilis (BsSecA), S. aureus (SaSecA1), P. aeruginosa (PaSecA) and B. anthraces (BaSecA1). Reconstituted liposomes were injected together with pOmpA and ATP-Mg2+ as described. When indicated, SecYEG at 0.47 ng and SecDF•YajC at 0.53 ng were used for reconstitution, which were the same amount as in SecA-depleted BA13 membranes; Re-13.: Reconstituted membranes after removing SecYEG from B13 membranes. n =20-30.
| SecAs | Lipo | Lipo +SecYEG |
L+SecYEG +SecDF•C |
BA13 memb |
RE-13 memb |
RE-13 memb +SecYEG |
RE-13 memb +SecYEG-DF•C |
|---|---|---|---|---|---|---|---|
| EcSecA | 0.4 ± 0.5 | 0.3 ± 0.1 | 3.8 ± 0.3 | 4.7 ± 0.1 | 0.4 ± 0.1 | 4.2 ± 0.3 | 4.4 ± 0.1 |
| BsSecA | 0.3 ± 0.2 | 3.1 ± 0.2 | 4.5 ± 0.2 | 5.8 ± 0.3 | 0.5 ± 0.5 | 5.0 ± 0.2 | 5.2 ± 0.3 |
| PaSecA | 0.3 ± 0.2 | 1.1 ± 0.2 | 2.0 ± 0.3 | 5.1 ± 0.2 | 0.3 ± 0.5 | 5.1 ± 0.2 | 5.1 ± 0.1 |
| SaSecAl | 0.4 ± 0.1 | 3.1 ± 0.2 | 4.2 ± 0.3 | 6.1 ± 0.3 | 0.5 ± 0.3 | 5.6 ± 0.4 | 5.0 ± 0.3 |
| BaSecAl | 0.3 ± 0.2 | 3.3 ± 0.2 | 4.0 ± 0.3 | 6.1 ± 0.3 | 0.5 ± 0.5 | 5.0 ± 0.2 | 5.3 ± 0.3 |