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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Biochim Biophys Acta. 2014 Oct 30;1853(1):222–232. doi: 10.1016/j.bbamcr.2014.10.019

Fig. 4.

Fig. 4

The mutation of ATP binding site in D1 or D2 domain of VCP-GFP decreases the nuclear retention. (A) HEK293 cells transfected with VCP-GFP mutant A1 or A2 were imaged using confocal microscopy. (B) Quantitative analysis of the nuclear retention. Data are presented as the fluorescence intensity ratio between the nucleus and the cytoplasm calculated using MIPAV software (n=36, * P<0.05).