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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Biochim Biophys Acta. 2014 Oct 30;1853(1):222–232. doi: 10.1016/j.bbamcr.2014.10.019

Fig. 5.

Fig. 5

The sequence 780-785 in the C-terminal region regulates VCP-GFP retention in the nucleus and nucleoli. HEK293 cells were transiently transfected with C-terminal region deletion mutant VCP-Δ780-806, VCP-Δ786-806 and VCP-Δ797-806 for 16 hr. The images were taken under an inverted fluorescence microscope. (A) The images showed that VCP-Δ780-806, but not VCP-Δ786-806 and VCP-Δ797-806, accumulates in the nucleus and forms foci as indicated by red arrows. (B) Quantitative analysis of the results presented in (A). Data are presented as the fluorescence intensity ratio between the nucleus and the cytoplasm calculated using MIPAV software (n=48, * p<0.01). (C) The VCP-Δ780-806 transfected cells were stained using Hoechst 33342 and imaged with fluorescence microscope and the images of the green fluorescence of VCP-Δ780-806 and the nucleus were merged (scale bar = 5 μM). (D) HEK293 cells expressing VCP-Δ780-806 and VCP-Δ786-806 mutants were fixed with cold methanol and stained with anti-nucleolin antibody and TRITC labeled secondary antibody. Images were taken using confocal microscopy (scale bar=5 μM).