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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Biochim Biophys Acta. 2014 Oct 30;1853(1):222–232. doi: 10.1016/j.bbamcr.2014.10.019

Fig. 8.

Fig. 8

in cis and in trans effect of the C-terminal sequence 780-806 on VCP-Δ1-206. Cells were transfected with VCP-Δ780-806 with control vector or VCP-Δ1-206, and VCP-Δ1-206 with control vector, or VCP-Δ1-206-Δ780-806 for 16 hr. (A) The cells were imaged with an inverted fluorescence microscope. (B) Fluorescence intensity ratio between the nucleus and cytoplasm (FIRNC) were calculated using Olympus CellSens Dimension software. The comparison of FIRNC between VCP-Δ780-806+VCP-Δ1-206 and VCP-Δ1-206 using ANOVA showed a significant difference (p<0.05, n=50). (C) Distribution of VCP deletion mutants in the nuclear and cytoplasmic fractions. HEK293 cells transiently transfected with VCP-GFP deletion mutants as indicated, and then fractionated into the nuclear fraction (N) and cytoplasmic fraction (C). VCP-GFP variants were detected by anti-GFP antibody. GAPDH and lamin A/C were used as cytoplasmic and nuclear markers, respectively.