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. Author manuscript; available in PMC: 2015 Nov 14.
Published in final edited form as: Biochem Biophys Res Commun. 2014 Oct 22;454(2):335–340. doi: 10.1016/j.bbrc.2014.10.073

Fig. 1.

Fig. 1

(A) DU145 and MDA-MB-231 cells were treated with siRNA targeting actin (siActin (+)), a non-targeting siRNA (siCon (−)) or mock treatment (M) for 48 or 96 h. (A) ITGA6 was immunoprecipitated from DU145 and MDA-MB-231 cells and immunobot analysis was used to detect ITGA6 and ITGA6p following a 96 h siRNA treatment. DU145 (B) and MDA-MB-231 (C) cells were harvested following 48 or 96 h of siRNA treatment and immunoblot analysis was performed to detect actin expression. Tubulin was used as a loading control. Flow cytometric analysis of cell surface ITGA6 in (D) DU145 and (E) MDA-MB-231 cells after 96 h of siRNA treatment. Secondary only is the grey filled peak, siActin is the black solid line, siCon is the grey line, and mock is the black dotted line. Independent experiments were performed three times.