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. Author manuscript; available in PMC: 2014 Dec 4.
Published in final edited form as: Exp Neurol. 2013 Mar 16;247:694–702. doi: 10.1016/j.expneurol.2013.03.010

Figure 2. Fetal CD140a/O4-defined OPCs drive Sox10-MCS5 enhancer activity.

Figure 2

Unsorted human fetal brain dissociates, 19–22 weeks gestational age, were infected with single Sox10 reporter virus 7–8 hours following plating. Multicolor flow cytometry performed was 3–4 days post-infection with CD140a and O4 (n=3, fetal brain preparations). A–B, Representative dot plots and cell-type incidence (%) showing GFP reporter expression (x-axis) plotted against CD140a-PE (A) or O4-APC (B) fluorescence (y-axes) (19 week, 10,000 events shown). C, mean GFP fluorescence intensity among individual CD140a/O4 defined cell populations following normalization to CD140a−O4− cell intensity (n=3, mean ± SEM). * indicates significantly higher GFP expression among CD140a+O4+ defined OPCs (p<0.05, Tukey’s multiple comparison test p-value vs. double negative cells).