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. 2014 Dec 3;9(12):e113790. doi: 10.1371/journal.pone.0113790

Figure 3. Putative TAS3c locus and the predicted targets of tasiRNAs in Chinese sacred lotus.

Figure 3

(a) The sequences of the three putative TAS3 loci in Chinese sacred lotus. The red and blue regions are 5′ and 3′ miR390 complementary sites, respectively. The regions of upper case nucleotides are mature tasiRNAs that target ARF family members, or tasiARFs. The underlined region of TAS3c are a phased siRNA TAS3_D6-2(+) with position 12. (b) The 5′ miR390 binding sites on TAS3 transcripts. Only the commonly aligned nucleotides are aligned to miR390. (c) The 3′ miR390 binding sites on TAS3 transcripts. (d) The mature tasiRNAs that target ARF family members derived from TAS3a/b/c loci. (e) The reads distribution and phase score of TAS3c. Legend are the same as those in Figure 2. The vertical dashed lines with distances of 21 nt are phase positions that start from position 10 of the 3′ miR390 site. (f) The predicted targets of tasiRNAs. The yellow and blue circle includes 7 and 8 ARF family members that are targeted by TAS3a/b and TAC3c derived tasiRNAs, respectively. (g) The complementary site of TAS3_D6-2(+) and an ARF family member, NNU_003220-RA.