Table 3.

Cell types and culture media

Cell	Medium
Human umbilical vein EC (HUVEC, Lonza, Basel, Switzerland)	EGM-2 medium (Lonza)
Rat aortic endothelial cells (RAEC, VEC Technologies, Rensselaer, NY)	Serum supplemented MCDB-131-based EC medium (VEC Technologies)
L929 cells (ATCC), transduced to produce luciferase, as described elsewhere[15]	Serum supplemented RPMI 1640 (Invitrogen, Burlington, ON)
NIH 3T3 cells (ATCC, Manassas, VA)	DMEM, (Invitrogen) supplemented with 10% calf serum (Sigma)
Cardiac cells, primary isolate from neonatal Sprague-Dawley rats according to a published protocol[22]	If no EC: DMEM (25 mM glucose and 0 mM sodium pyruvate, Gibco) with 10% bovine serum (Sigma) and 1% Hepes (Gibco). With EC: a co-culture medium composed of DMEM with 5.5 mM glucose and 1.28 mM sodium pyruvate supplemented with 10% bovine serum (Sigma)

All medium were also supplemented with 1% penicillin/streptomycin (Lonza).