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. 2014 Dec 3;9(12):e113785. doi: 10.1371/journal.pone.0113785

Figure 5. PDGFRα interacts with BMPRIA.

Figure 5

A. PDGFRα (His tagged) was co-expressed with BMPRIA(HA tagged) in 293T cells. The cells were treated with 25 ng/ml PDGF-AA for 0.5 or 2 h or untreated. Cell lysates were divided into two parts for analysis of PDGFRα and BMPRIA expression by western blot, or immunoprecipitation of PDGFRα to test whether BMPRIA was brought down with PDGFRα. B. PDGFRα (His tagged) was co-expressed with BMPRIA(HA tagged) in 293T cells. The cells were treated with 25 ng/ml PDGF-AA for 0.5 or 2 h or untreated. Cell lysates were divided into two parts for analysis of PDGFRα and BMPRIA expression by western blot, or immunoprecipitation of BMPRIA to test whether PDGFRα was brought down with BMPRIA. C. Co-immunoprecipitation of endogenous PDGFRα and BMPRIA. Primary MSC cells were lyzed and cleaned lysates were incubated with IgG, anti-BMPRIA antibodies, or anti-PDGFRα antibodies overnight at 4°C. The immunoprecipitated proteins and their interacting proteins were detected by western blot.