Table 1. Gene expression analyzed by qRT-PCR and RNA-Seq.
Gene name | Unigene ID | Primer name | Primer sequences | Fold change (MA:MB) | |
RNA-Seq | qRT-PCR | ||||
AVP | comp51265_c0_seq1 | AVP-F | GTGCTACGCTCGTCATCTCG | Inf | Inf |
AVP-R | CAGTAGAACGCACGGATTGG | ||||
ATP6V1A | comp25755_c0_seq1 | ATP6V1A -F | AGACTGTCAAGGACGGTAAG | Inf | 126 |
ATP6V1A -R | CCAGCTCTTTCGTAGAACTG | ||||
RAB1A-1 | comp8328_c0_seq1 | RAB1A-1F | CGGAAAGTCGTGTCTGCTGT | Inf | Inf |
RAB1A-1R | CGTTCTCACAAGCATATCGGTC | ||||
SESB | comp12445_c0_seq1 | SESB-F | GTTACCACGCCAGAACGAGC | Inf | 153 |
SESB-R | CCACCTCGGACAACAGCAAC | ||||
GLYS | comp45386_c0_seq1 | GLYS -F | GCTGTCACCAAGTCTTTACG | 0.365 | 0.400 |
GLYS -R | GGTCCACAGCATCGTCACAC | ||||
GLDC | comp38932_c0_seq2 | GLDC -F | CTCAAGAGCATCGCCAATCG | 0.271 | 0.188 |
GLDC -R | TCCACAGTCTCGTCCAATGC | ||||
LEA2 | comp29147_c0_seq1 | LEA2-F | AGCGTCTAGCAGGATTGGTC | 0 | 0 |
LEA2-R | GATTCGGAAGGTCCCACGAC | ||||
PCE | comp35865_c0_seq1 | PCE-F | GGTTGCCAGAGCCCTCATAC | 8.863 | 6.122 |
PCE-R | CTCGTGCCGTGCTGACAGAA |
‘Inf’ means that the genes were only expressed in MA.