Table 1.
Gene Name, Forward and Reverse Primers | Primer Sequence | Accession No. | Expected Size, base pairs | Exons Spanned |
---|---|---|---|---|
Gapdh-F | TGAACGGATTTGGCCGTATTG | NM_008084 | 200 bp | 2–3 |
Gapdh-R | GATGGGCTTCCCGTTGATGA | |||
Pdgfra-F | ATGACAGCAGGCAGGGCTTCAACG | NM_011058 | 195 bp | 4–5 |
Pdgfra-R | CGGCACAGGTCACCACGATCGTTT | |||
Myh11-F | CTACACTGCGCAATACCACG | NM_013607 | 200 bp | 16–17 |
Myh11-R | TGCCAGGATCTCATAGCGTT | |||
Kit-F | CGCCTGCCGAAATGTATGACG | NM_021099 | 162 bp | 19–21 |
Kit-R | GGTTCTCTGGGTTGGGGTTGC | |||
Uchl1-F | CGATGGAGATTAACCCCGAGATG | NM_011670 | 169 bp | 1–4 |
Uchl1-R | TTTTCATGCTGGGCCGTGAG | |||
Gucy1a3-F | TGTTCACCTCTGCAGGTCAT | NM_021896 | 115 bp | 6–7 |
Gucy1a3-R | CCACACAATATGCATCCCCG | |||
Gucy1b3-F | GATCCGCAATTATGGTCCCG | NM_017469 | 171 bp | 2–4 |
Gucy1b3-R | AACATCTGCAGGATTTCGCC | |||
Prkg1-F (primer 1) | TATCATCAGGCAGGGTGCAA | NM_011160 | 192 bp | 6–7 |
Prkg1-R (primer 1) | GACAGCTTCTGCGGCAATAA | |||
Prkg1-F (primer 2) | AGGGGGCTCATTCTGACTTC | NM_011160 | 178 bp | 11–13 |
Prkg1-R (primer 2) | GGCGAATGCTTCTACCACAC |