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. 2014 Oct 9;307(11):G1057–G1072. doi: 10.1152/ajpgi.00331.2014

Table 1.

Primer sequences used for quantitative PCR

Gene Name, Forward and Reverse Primers Primer Sequence Accession No. Expected Size, base pairs Exons Spanned
Gapdh-F TGAACGGATTTGGCCGTATTG NM_008084 200 bp 2–3
Gapdh-R GATGGGCTTCCCGTTGATGA
Pdgfra-F ATGACAGCAGGCAGGGCTTCAACG NM_011058 195 bp 4–5
Pdgfra-R CGGCACAGGTCACCACGATCGTTT
Myh11-F CTACACTGCGCAATACCACG NM_013607 200 bp 16–17
Myh11-R TGCCAGGATCTCATAGCGTT
Kit-F CGCCTGCCGAAATGTATGACG NM_021099 162 bp 19–21
Kit-R GGTTCTCTGGGTTGGGGTTGC
Uchl1-F CGATGGAGATTAACCCCGAGATG NM_011670 169 bp 1–4
Uchl1-R TTTTCATGCTGGGCCGTGAG
Gucy1a3-F TGTTCACCTCTGCAGGTCAT NM_021896 115 bp 6–7
Gucy1a3-R CCACACAATATGCATCCCCG
Gucy1b3-F GATCCGCAATTATGGTCCCG NM_017469 171 bp 2–4
Gucy1b3-R AACATCTGCAGGATTTCGCC
Prkg1-F (primer 1) TATCATCAGGCAGGGTGCAA NM_011160 192 bp 6–7
Prkg1-R (primer 1) GACAGCTTCTGCGGCAATAA
Prkg1-F (primer 2) AGGGGGCTCATTCTGACTTC NM_011160 178 bp 11–13
Prkg1-R (primer 2) GGCGAATGCTTCTACCACAC