Fig. 2.

Effect of nitrite therapy on indices of acute lung injury. Male mice were exposed to Cl₂ 600 ppm, 45 min and then brought back to room air and 30 min thereafter received saline or nitrite 10 mg/kg by IM injection. Mice were continually observed over the next 6 h, and, if they died during this time, bronchoalveolar lavage fluid (BALF) and blood were collected immediately. At 6 h, all mice were killed, and BALF and plasma were collected. A and B: BALF levels of protein and total cell accumulation. *P < 0.01 relative to air, #P < 0.05 relative to Cl₂ alone by 1-way ANOVA with Tukey's posttest. C: differential cell analysis. D: viability of BALF inflammatory cells. *P < 0.01 relative to respective + nitrite group by t-test. E: BALF and plasma macrophage inflammatory protein 2 (MIP-2) levels, *P < 0.01 relative to air and #P < 0.05 relative to Cl₂-alone group by 1-way ANOVA with Tukey's posttest. F: BALF and plasma keratinocyte chemokine (KC) levels, *P < 0.01 or #P < 0.05 relative to air, by 1-way ANOVA with Tukey's posttest. All data are means ± SE (n = 5–9).