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. 2014 Oct 17;307(11):L868–L876. doi: 10.1152/ajplung.00096.2014

Fig. 3.

Fig. 3.

Conditional depletion of SMC EC-SOD augments chronic hypoxia-induced vascular medial wall remodeling. A: representative lung α-smooth muscle actin (α-SMA) immunostaining (brown signal) with counterstain by hematoxylin (blue) after 35 days of hypoxia or normoxia in CO and KO mice. Bar = 200 μm. Arrows indicate muscularized small vessels identified by positive α-SMA immunostaining. B: quantitation of muscularized small vessels (<50 μm) in a ×10 high-power field (HPF) in normoxia and after 35 days of hypoxia. Data are expressed as no. vessels/HPF, n = 6 mice/group. *P < 0.05 vs. CO Norm; #P < 0.05 vs. CO Hypo. C: representative lung α-SMA immunostaining of a midsized pulmonary artery used to determine medial wall thickening. Bar = 60 μm. D: quantitation of medial wall thickness (MWT) of pulmonary arteries adjacent to terminal bronchioles (<200 μm). Data expressed as no. of vessels/HPF, n = 5–11 mice/group and 4–9 vessels/mouse. *P < 0.05 vs. CO Norm.