Fig. 9.

l-Arg or l-Cit supplementation did not restore renal inducible nitric oxide synthase (iNOS) or neuronal nitric oxide synthase (nNOS)-β protein expression in diabetic mice. Kidney iNOS (A) and nNOS-β (B) protein expression in tissue lysates was detected by Western blot analysis after 9 wk of STZ-induced diabetes. β-Actin or GAPDH was used as a loading control protein, and brain tissue lysate was used as a positive control for nNOS-α in B. Quantification was performed by densitometry followed by normalization to β-actin or GAPDH. Data are presented as means ± SE; n = 4–5 mice/group. *P < 0.05 compared with the normal group.