Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Sep 18;307(11):F1274–F1282. doi: 10.1152/ajprenal.00213.2014

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2014 the American Physiological Society

PMC Copyright notice

Fig. 4. — Distribution in epithelial, endothelial, and interstitial compartments of the kidney. A, top panels: representative hpf images of immunostaining experiments; highlighted regions are shown magnified in the bottom panel of images. Deoxyuridine-labeled cells are shown in green, epithelial or endothelial labeling is shown in red as indicated, and nuclei are stained with DAPI (blue) in all images. A–F: representative images of early neonatal (A and D), late neonatal (B and E), and adult (C and F) LRCs with the epithelia marked by antibody to Na-K-ATPase (A–C; cortex) or a cocktail of antibodies (D–F; papilla). G–I (papilla) are representative images of early neonatal (G), late neonatal (H), and adult (I) LRCs with vascular endothelia marked by antibody to MECA-32 (red). In the bottom panel of images, representative positive nuclei within the labeled nephron region for each corresponding labeling paradigm are indicated by thin white arrows (early neonatal LRC), thick white arrows (late neonatal LRC), or white arrowheads (adult LRC). The scale bars in A–I represent 200 μm. B, bottom panels: graphical representation of the percentage distribution of different populations of LRCs in epithelial, endothelial, and interstitial compartments. Statistical significance (P < 0.0001) is indicated by an * in the graphs. Comparisons that were not statistically significant are indicated as NS.

Fig. 4. — Distribution in epithelial, endothelial, and interstitial compartments of the kidney. A, top panels: representative hpf images of immunostaining experiments; highlighted regions are shown magnified in the bottom panel of images. Deoxyuridine-labeled cells are shown in green, epithelial or endothelial labeling is shown in red as indicated, and nuclei are stained with DAPI (blue) in all images. A–F: representative images of early neonatal (A and D), late neonatal (B and E), and adult (C and F) LRCs with the epithelia marked by antibody to Na-K-ATPase (A–C; cortex) or a cocktail of antibodies (D–F; papilla). G–I (papilla) are representative images of early neonatal (G), late neonatal (H), and adult (I) LRCs with vascular endothelia marked by antibody to MECA-32 (red). In the bottom panel of images, representative positive nuclei within the labeled nephron region for each corresponding labeling paradigm are indicated by thin white arrows (early neonatal LRC), thick white arrows (late neonatal LRC), or white arrowheads (adult LRC). The scale bars in A–I represent 200 μm. B, bottom panels: graphical representation of the percentage distribution of different populations of LRCs in epithelial, endothelial, and interstitial compartments. Statistical significance (P < 0.0001) is indicated by an * in the graphs. Comparisons that were not statistically significant are indicated as NS.