Fig. 5.

Distribution of LRCs in epithelial compartments of the nephron. A, top panels: representative images of LRCs colabeled with lotus tetragonolobous lectin (A–C; red; cortex), antibody to aquaporin-2 (D–F; red; papilla) to identify proximal tubule and collecting duct, respectively. Scale bars represent 200 μm. In the bottom panel of images, highlighted regions of the corresponding panels are shown; nuclei within the epithelial compartment that is positive for the deoxyuridine label are indicated by thin white arrows (early neonatal LRC), thick white arrows (late neonatal LRC), or white arrowheads (adult LRC). Dark staining with antibody to Na-K-ATPase (not shown; see Fig. 4) was used to identify the distal tubule. B, bottom panels: graphs in G–I indicate the percentage distribution of each LRC population within nephron regions. Statistical significance (P < 0.0001) is indicated by an * in the graphs. Comparisons that were not statistically significant are indicated as NS.