Fig. 3.
Cofractionation of D1-like dopamine receptors and α1A-ARs in hRPTCs. Serum-starved (1 h) hRPTCs were treated with Fen (1 μM, 15 min) or with methyl-β-cyclodextrin (β-CD) to disrupt lipid raft microdomains. PM-enriched cellular pellets were then subjected to sucrose gradient ultracentrifugation, and 12 fractions were obtained. The most buoyant fractions (fractions 1–6) represent lipid rafts, whereas the rest (fractions 7–12) represent nonlipid rafts. The various fractions were then probed for proteins of interest. Caveolin-1 was used as a marker for lipid raft microdomains. n = 4.