Fig. 4.
Protein expression profiles after D1-like dopamine receptor treatment in hRPTCs. Serum-starved (1 h) hRPTCs were treated with Fen (1 μM) at the indicated time points. A–C: PM-enriched fractions were prepared for immunoblot analysis using uniform amounts of protein. Pan (p)-cadherin was used for normalization. D–F: in a separate set of experiments, Fen-treated hRPTCs were labeled with cell membrane-impermeant sulfo-NSS-SS-biotin, and surface proteins were isolated using an avidin column and then probed for D1Rs, D5Rs, and α1A-ARs. Numeric data are expressed as means ± SE. Representative blots are shown above the graphs. *P < 0.05 vs. the other groups (by one-way ANOVA and a Holm-Sidak post hoc test). n = 3–4/group.