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Immunostaining of key proteins of neurosteroidogenesis in the 21-day-old rat male CER. Immunostaining of StAR, aromatase, ER receptor, LHR, and SF-1-positive cells was performed on sagital brain sections (n = 5/group). The cell nuclei were counterstained by the fluorescent dye DAPI (blue). No significant difference of expression was observed between control and deficient pups from dams fed the deficient diet.
