Table 3.

Circulating HCY and cerebellar concentration of folate, vitamin B12, SAM, and SAH measured in 21-day-old rats from controls and dams fed the methyl donor-deficient diet during pregnancy and lactation periods

	Females		Male
	Normal diet	Deficient diet	Normal diet	Deficient diet
HCY, μmol/l	9.27 ± 0.91	39.24 ± 21.78**	9.45 ± 1.03	30.24 ± 14.11**
Vitamin B9, nmol/g tissue	0.92 ± 0.12	0.32 ± 0.19**	0.89 ± 0.24	0.32 ± 0.19*
Vitamin B12, pmol/g tissue	44.8 ± 11.34	21.76 ± 2.05**	26.63 ± 6.43	18.00 ± 2.12*
SAM, nmol/g tissue	26.12 ± 9.00	26.23 ± 9.94	31.77 ± 4.24	31.13 ± 9.92
SAH, nmol/g tissue	4.55 ± 1.05	8.33 ± 2.02**	4.88 ± 0.84	6.38 ± 1.96
SAM/SAH ratio	5.82 ± 1.92	3.36 ± 1.63*	6.70 ± 1.62	5.30 ± 2.30

Data are means ± SD; n = 5 in each group. SAM, S-adenosylmethionine; SAH, S-adenosylhomocysteine. Statistically significant differences between control and deficient animals for each sex (ANOVA).

^*P < 0.05 and

^**P < 0.01 (ANOVA).