Fig. 2.

Postweaning body weight (BW), glucose, and insulin tolerance tests in kiss-p110α/β-KO and littermate control mice (n = 7–11). Deletion of p110α and p110β in kisspeptin cells did not affect postweaning BW in males (A) or females (B). WT and kiss-p110α/β-KO showed similar glucose (C and D) and insulin (E and F) sensitivity. Calculated areas under the curve (AUC) are shown as insets. Adult (2- to 3-mo-old) chow-fed mice were used for glucose and insulin tolerance tests. Results are expressed as mean glucose concentration ± SE. G: baseline and insulin-stimulated p-Akt in the liver of WT and kiss-p110α/β-KO males. Liver lysates from saline and insulin-stimulated male mice were blotted with antibodies against p-Akt (Ser⁴⁷³). The blots were then stripped and reprobed with antibodies to total Akt. Densitometry analysis (normalized to total Akt) is shown in the adjacent bar graph; the values are means ± SE expressed as arbitrary units.