Fig. 6.

COOH terminus of HSC70-interacting protein (CHIP) has no effect on FSP27 stability. 3T3-L1 adipocytes were transfected with control or CHIP-specific siRNA for 1 day and then infected with Ad-FSP27-FLAG. Two more days later, cells were treated with 10 μg/ml CHX (A and B) or 2 mM AICAR (C and D) for the indicated times. FSP27-FLAG was detected by IB, using β-actin as a loading control (A and C), and subjected to densitometric measurement (B and D). Data are presented as the mean ± SD of 3 independent experiments.