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. 2014 Dec 3;9(12):e114348. doi: 10.1371/journal.pone.0114348

Figure 5. Bpa acts as a proteasomal activator.

Figure 5

A, Peptidase activity measured by fluorescence detection of AMC molecules (λex: 360 nm, λem: 460 nm) released from the fluorogenic peptide L-Suc-LLVY-AMC (150 µM). PrcAB exhibits a turnover number (TON) of 0.057±0.004 s−1, which is more than three times less active than Δ7PrcAB with a TON of 0.185±0.014 s−1. Addition of an excess of Bpa to the reaction increases peptidase activity of PrcAB about 1.8 fold to a TON of 0.105±0.008 s−1. B, Degradation of β-casein (27 µM) at 37°C by Δ7PrcAB or PrcAB (0.2 µM) in presence or absence of Bpa or BpaΔHbYX (6 µM protomer) is sampled at different time points and analyzed by Coomassie-stained SDS-PAGE.