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. 2014 Sep 26;307(11):H1675–H1684. doi: 10.1152/ajpheart.00548.2014

Fig. 3.

Fig. 3.

Liver and heart levels of lipids for age- and genetic background-matched chow-fed male WT and Bco1−/− mice. Liver triglyceride (TG; A) and total nonesterified fatty acids [free fatty acid (FFA); B] concentrations were not different for WT and Bco1−/− mice, whereas heart TG (C) concentrations were diminished and total FFAs (D) were elevated in Bco1−/− mice compared with WT mice. A number of relatively abundant individual FFA species were also significantly elevated in Bco1−/− hearts (E). Expression of peroxisome proliferator-activated receptor (PPAR)-γ (Pparg), lipoprotein lipase (Lpl), comparative gene identification 58 (Cgi-58), and Cd36 mRNAs but not PPAR-α (Ppara), hormone-sensitive lipase (Hsl), or adipocyte triglyceride lipase (Atgl) were significantly elevated in Bco1−/− hearts (F). Elevated expression for genes involved in FFA synthesis and metabolism, including stearoyl-CoA desaturase 1 (Scd1), fatty acid synthase (Fas), and diacylglycerol O-acyltransferase 2 (Dgat2), were also elevated in Bco1−/− hearts (G). Total ceramide (H) and total N-acylethanolamine (NAE; I) concentrations were also significantly elevated in hearts of Bco1−/− mice. Values are means ± SE; n = 5–8. *P < 0.05; **P < 0.01.