Fig. 7.

sGC heterodimer containing α1-isoD has a diminished activation capacity. A: domain organization of canonical α1 and α1-isoD variants. α-N term., NH₂-terminal region; PAS, Per/Arnt/Sim domain; CC, coiled coil domain; CAT, catalytic domain. B: fold activation of cGMP-forming activity in lysates of COS7 cells ectopically expressing α1/β1 and α1-isoD/β1 heterodimers. Data are means ± SD from three independent experiments performed in triplicates. The P values for α1/β1 vs. α1-isoD/β1 sGC activities for each treatment are indicated.