Table 2.
Functionality and detection of human α1 sGC (GUCY1a3) and β1 (GUCY1b3) sGC transcripts
| Transcript, NCBI Acc. No. | Detected in Aorta | Isoform/functional | Primers | Targeted Sequence | Product Length, bp |
|---|---|---|---|---|---|
| α1 sGC splice isoforms | |||||
| Transcript 1 NM000856 | + | Isoform A/Yes | Fr1: GAAAGCGTGAGCAGGGGGCC | Unique exon 3 | Tr1: 174 |
| Rv1: ACCCTGCTGCGATCTCCTCCT | |||||
| Transcript 2 NM001130682 | − | Isoform A/Yes | No specific sequences, no primers | ||
| Transcript 3 NM001130683 | − | Isoform A/Yes | Fr3: ACACCTGGAACAAGAGCAGCAAA | Exon 1 shared with Tr 5 | Tr3: 155 |
| Rv3: AGGGAAGACGGCAGCTCACTGT | |||||
| Transcript 4 NM001130684 | − | Isoform A/Yes | Fr4: TGGGCCCCAGAGCCCATCAG | Unique exon 1 | Tr4: 259 |
| Fr4: ACCCTGCTGCGATCTCCTCCT | |||||
| Transcript 5 NM001130685 | + | Isoform B//Yes# | Fr5: CAGCCCCGAGGTGTGCGAAG | Deletion in first coding exon | Tr 2,3,6,7,8: 273 Tr 5: 94 |
| Rv5: GGCACGGTTGCTTTGCAGCT | |||||
| Transcript 6 NM001130686 | + | Isoform C/Dominant negative | Fr6: ACCAGACGTTTAGCGGGATCA | Rv primer in unique 3′ end of last exon | Tr6: 192 |
| Rv6: ATCAAACATTTGAAGTGATGCATATA | |||||
| Transcript 7 NM001130687 | + | Isoform D/No activation# | Fr7: AGTTATGTCTCCCCATGGAGAACCT | Rv primer in unique 3′ end of last exon | Tr7: 208 |
| Rv7: AGGTGTTTAACATAATTACTACCTGTAAG | |||||
| β1 sGC splice isoforms | |||||
| Transcript 1 AK300296 | + | Isoform 1/TBD, likely no activation | Fr10: ACAATCTTGCGTGTCCTGGGCTC | In frame insertion of exon 3′ | Tr2: 217 |
| Rv10: GTGCCACTGTTTTGATGATGCCCT | Tr1: 151 | ||||
| Transcript 2 NM000857 | + | Isoform 2/Yes | Fr8: CCTCTGCATGATGCCACGCG | Sequence in exon 10 | Tr2: 300 |
| Rv8: TCTGGTGTAGAGGTCGTTGAGGAGG | |||||
| Transcript 3 AK296680 | − | Isoform 3/TBD, likely no activation | Fr9: CGTACCTCTGCGTGGGGGCT | In frame unique exon 4′ insertion | Tr2: 226 |
| Rv9: TTGAGGACTTTGCTTGCAGCAGCA | Tr3: 378 | ||||
| Transcript 4 AK307838 | + | Isoform 4/TBD, likely no activation | Fr11: GTACAAACGACACTGATGCTGTGTG | Insertion in exon 10 | Tr4: 300 |
| Rv11: CAAATGGGTTTTTCCGGGAATCAGTC | |||||
| Transcript 5 AF020340 | − | Isoform 5/TBD, likely no activation | Fr8: CCTCTGCATGATGCCACGCG | Deletion in exon 10 | Tr2: 300 |
| Rv8: TCTGGTGTAGAGGTCGTTGAGGAGG | Tr5: 240 | ||||
| Transcript 6 AK299916 | + | Isoform 6/TBD, likely no activation | Fr12: GCCAGCGGAAGGGAAGGCTCC | Unique alternative exon 1 | Tr6: 150 |
| Rv12: TTGATGTCTTCCCACACCTCGGGGC | |||||