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. 2014 Dec 3;9(12):e114379. doi: 10.1371/journal.pone.0114379

Figure 5. Electrophoretic examination of the tested bile salt hydrolases.

Figure 5

SDS-PAGE analysis of fractions obtained during the purification of BSHs from B. pseudocatenulatum (A) and B. suis (B) was performed on 12% polyacrylamide gels under denaturing conditions. Gels were stained with Coomassie Brilliant Blue R250. Lanes: 1, molecular weight marker; 2, cell-free extract; 3, pooled fractions from hydrophobic interaction chromatography (HIC); 4, active fractions from HIC plus ion-exchange chromatography. Isoelectric point determination of the purified BSHs from B. pseudocatenulatum (C) and B. suis (D) was performed using 7 cm, pH 3-6, linear IPG strips stained with CBB R250.