Figure 4. The CuO NP affects viability of human BEAS-2B lung cells.

(A–D) BEAS-2B cells were plated on 96-well plates, left to recover for one day and then exposed to the CuO NP (30–50 nm) for 24 h at concentrations displayed on the graph. The cell viability was measured by the Alamar Blue assay and the statistical analysis (T-test) was done using SigmaPlot. The p values are displayed for the concentrations with statistically significant changes. CuO NP concentrations are displayed at different scales for readability. (E) BEAS-2B cells were plated on 96-well plates, left to recover for one day and then exposed to the 0.01 µg/cm² CuO NP (30–50 nm) for 0 h, 24 h, 48 h and 72 h. Cell viability was measured by the Presto Blue assay.