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. Author manuscript; available in PMC: 2014 Dec 4.
Published in final edited form as: Oncogene. 2013 Jan 14;33(4):525–531. doi: 10.1038/onc.2012.597

Figure 2. DMAP1-deficient cells are radiosensitive.

Figure 2

A549 cells were transfected with DMAP1 or control siRNA, and after 48 hours were irradiated at the doses indicated. Cells were immediately incubated in the presence of either (a) DMSO or (b) nocodazole and after 18 hours fixed and analysed for phospho-serine 10-Histone 3 (pH3) by FACS analysis. In (c) A549 cells were transfected with siDMAP1 or control siRNA. After 48 hours, DMSO or ATM inhibitor (ATMi) was added, and one hour later cells were treated with the indicated radiation doses and counted. 1000 cells were plated per condition, and the number of colonies formed was counted seven days later. At least three independent experiments were performed and p values calculated using the student’s t-test, (*p<0.05; **p<0.01).