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. Author manuscript; available in PMC: 2014 Dec 4.
Published in final edited form as: Oncogene. 2013 Jan 14;33(4):525–531. doi: 10.1038/onc.2012.597

Figure 3. DMAP1 is required for non-canonical ATM signalling.

Figure 3

(a) HCT-116 cells were transfected with a pool of siRNAs against DMAP1 or control siRNA, then 48 hours later were treated with 135mOsm NaCl for one hour. (b) HCT-116 cells were transfected with Myc-DMAP1, and 48 hours later treated with 2 Gy IR or 135mOsm NaCl, then harvested one hour later. (c) Cells were pre-treated for one hour with ATM inhibitor (ATMi) or DMSO, and treated with 50 J m−2 UV. Whole cell lysates were immunoblotted for pATM, ATM, pSMC1, SMC1, pKAP1, KAP1, phospho-p53, p53, pChk1, Myc, actin or tubulin. * denotes a non-specific band.