Fig. 4.

Reduction in salt stress tolerance upon inhibition of BIN2. (A) Morphology of seedlings treated with mock or various salts. Seedlings were treated separately and arranged in a new plate to obtain a collective image of responses. First row, mock-treated control. Second row, treatment with KCl or LiCl alone. Third row, NaCl treatment in combination with KCl or LiCl. Scale bar = 5 mm. (B, C) Survival rate of Arabidopsis seedlings grown in 150 mM (B) or 200 mM NaCl media (C) supplemented with salts or bikinin (a BIN2 inhibitor). Survival rate was measured as the ratio of the number of green vs. pale yellow seedlings. Error bar = SD (n = 3). (D) GUS staining pattern in the roots of RD26pro:GUS seedlings. Scale bar = 1 mm.