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. Author manuscript; available in PMC: 2015 Dec 15.
Published in final edited form as: Bioorg Med Chem Lett. 2014 Nov 7;24(24):5568–5571. doi: 10.1016/j.bmcl.2014.11.010

Figure 3.

Figure 3

Inhibition of HLCS by biotin β-ketophosphonate-5′-AMP. (A) HLCS activity was quantified using infrared absorbance in the presence of 20 μM biotin and various concentrations of biotin β-ketophosphonate-5′-AMP (One way-ANOVA; *P<0.05; N=4; Dunnett’s multiple t-test *p<0.05, **p<0.01, ***p<0.001). (B) Representative gel, depicting biotinylation of p67 in the presence of various concentrations of biotin β-ketophosphonate-5′-AMP. Lane 6 shows p67 incubated in the absence of HLCS. (C) Competitive inhibition of HLCS by biotin β-ketophosphonate-5-AMP.