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. Author manuscript; available in PMC: 2015 Dec 2.
Published in final edited form as: Structure. 2014 Nov 6;22(12):1735–1743. doi: 10.1016/j.str.2014.09.010

TABLE 1.

Summary of 15N relaxation and ITC data for PKA-CWT and PKA-CY204A.

PKA-CWT:ATPγN PKA-CY204A: ATPγN
Kd for PKI5-24 (μM)a 0.12 ± 0005 14.6 ± 2.1
Average R1 (s−1) 0.51 ± 0.08 0.51 ± 0.06
Average R2 (s−1) 51 ± 5 46 ± 4
Average HX-NOEb 0.79 ± 0.13 0.81 ± 0.12
kex from CPMG (s−1)c 1020 ± 150 NDd
pclosed (%)c 93.5 ± 8.5 NDd
a

Determined by ITC. Additional results found in Table S1.

b

Residue specific HX-NOE values found in Figure S5.

c

Complete group fit parameters found in Table S2.

d

Dynamics are no longer concerted; therefore overall parameters are not available.