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. Author manuscript; available in PMC: 2015 Dec 2.
Published in final edited form as: Cell Metab. 2014 Dec 2;20(6):991–1005. doi: 10.1016/j.cmet.2014.11.001

Figure 3. Time-restricted feeding reduces whole body fat accumulation and associated inflammation.

Figure 3

A. Representative H&E stained histological sections of epididymal white adipose tissue (eWAT; upper panels) and brown adipose tissue (BAT; lower panels) in the different feeding conditions, as indicated. Arrowheads point to crown structures.

B. Serum leptin concentration. The number of mice (n) analyzed per group was (i) n=10, (ii) n=8, (iii) n=6, and (iv) n=7.

C. Quantitative polymerase chain reaction (qPCR) analysis of selected pro-inflammatory cytokines (TNFa, IL10, IL1) and chemokine (Ccl8) mRNA expression in (i) eWAT and (ii) BAT. N= pool of 6-8 samples per feeding group. See methods for details.

D. Hepatic triglyceride content. (i) n=6, (ii-iv) n=12.

E. Serum triglyceride concentration. (i) n=10, (ii-iv) n=6.

Data are presented as mean ± SEM. t-test, *p < 0.05, **p < 0.01, ***p < 0.001.