Table 2.

Mtb H37Rv genes involved in the biogenesis of trehalose mono- and di-mycolates, sulfolipids, di- and poly-acyltrehaloses and mannosyl-β-1-phosphomycoketides.

Gene number	Gene name	Function	Evidence	Reference
Rv0129c	fbpC	Mycolyltransferase (antigen 85C)	E,P	[147–149]
Rv0206c	mmpL3	Inner membrane transporter of the RND superfamily involved in the translocation of TMM	P	[145, 146]
Rv0295c	Sft0	Sulfotransferase responsible for the formation of the trehalose-2-sulfate moiety of sulfolipids	E,P	[164]
Rv0757-Rv0758	phoP-phoR	Two-component transcriptional regulator involved in the regulation of SL, DAT and PAT	P	[143, 176]
Rv1180-Rv1181	pks3-pks4	Polyketide synthase responsible for the elongation of the methyl-branched mycosanoic and mycolipenic acids found in DAT, TAT and PAT	P	[196, 197]
Rv1182	papA3	Acyltransferase catalyzing the sequential transfer of the first straight-chain saturated fatty acyl chain followed by the first mycolipenoyl group onto the 2- and 3-positions of trehalose, respectively, in the biosynthesis of DAT and PAT	E,P	[199]
Rv1183	mmpL10	Inner membrane transporter of the RND superfamily thought to be involved in the translocation of DAT and PAT	H	-
Rv1184c	chp2	Acyltransferase thought to catalyze the last three acylations leading to the formation of PAT from DAT	H	-
Rv1185c	fadD21	Putative fatty acid AMP ligase providing Pks3/4 with activated long-chain fatty acid starter units	H	-
Rv1662-Rv1663	pks8-pks17	Polyketide synthase responsible for the elongation of the monomethyl-branched unsaturated C16 to C20 fatty acids found in DAT and PAT	P	[162]
Rv1886c	fbpB	Mycolyltransferase (antigen 85B)	E	[147, 149]
Rv2048c	pks12	Polyketide synthase involved in the elongation of the alkyl backbone of mycoketides	E,P	[218, 220]
Rv3416	whiB3	Regulator of SL, DAT and PAT synthesis	P	[174]
Rv3804c	fbpA	Mycolyltransferase (antigen 85A)	E,P	[147, 149, 152]
Rv3820c	papA2	Acyltransferase catalyzing the transfer of the first straight-chain saturated fatty acyl chain onto trehalose-2-sulfate in the biosynthesis of sulfolipids	E,P	[165]
Rv3821	sap	Integral membrane protein thought to facilitate the translocation of SL-1 to the cell surface	P	[168]
Rv3822	chp1	Acyltransferase catalyzing the acylation at the 6- and 6’-positions of sulfolipids	E,P	[168]
Rv3823c	mmpL8	Inner membrane transporter of the RND superfamily involved in the translocation of sulfolipids	P	[168, 170, 171]
Rv3824c	papA1	Acyltransferase catalyzing the transfer of the first (hydroxy)phthioceranoyl group at the 3’-position of the product of PapA2	E,P	[165]
Rv3825c	pks2	Polyketide synthase responsible for the elongation of the methyl-branched phthioceranic and hydroxyphthioceranic acids found in sulfolipids	P	[166]
Rv3826	fadD23	Putative fatty acid AMP ligase providing Pks2 with activated long-chain fatty acid starter units	H	-

E,P,H: see Table 1.