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(a) Vertical deflection image of a live cell in exponential phase showing early signs of septal cleavage. (b) Height profile of the line shown in a; arrows indicate ridges. (c) Image from panel a, showing the area selected for force mapping. (d) Force map (300 nm2). (e) Histogram of stiffness measured on the ridges, the interconnecting material, and the older cell wall (bin size = 0.0005 Nm−1). Black inset scale bars (a–c) represent 500 nm.
