Figure 1. Drug sensitivity following ncRNA.1343 deletion is due to increased tgp1⁺ expression.

(a) Schematic representation of genes flanking ncRNA.1343. (b) RT–qPCR experiments measured transcript levels for nearby genes in wild-type cells and following replacement of ncRNA.1343 with ura4⁺ (1343Δ::ura4⁺) or deletion (1343Δ). Error bars represent s.e.m. resulting from at least three independent replicates. (c) Northern analysis of tgp1⁺ transcript levels in wild-type and 1343Δ cells grown in the presence of phosphate. (d) Serial dilutions of wild-type, 1343Δ::ura4⁺, 1343Δ and tgp1Δ1343Δ double mutant spotted on non-selective YES medium or in the presence of TBZ (20 μg ml⁻¹), HU (10 mM) or caffeine (15 mM), respectively.