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. 2014 Nov 27;5:5576. doi: 10.1038/ncomms6576

Figure 2. Two distinct lncRNAs are transcribed from a bidirectional promoter upstream of tgp1+.

Figure 2

(a) Previously published strand-specific RNA-Seq analysis (Rhind et al.,31) upstream of SPBC1271.09/tgp1+, represented as reads per kilobase per million (RPKM). Location of qPCR primer pairs and probes for Northern analysis are shown below. (b) Rbp1 ChIP–qPCR experiments performed in wild-type cells. (c,e,g) Northern analysis of nc-1343, nc-tgp1 and tgp1+ transcript levels in wild-type, rrp6Δ, mmi1Δ and 1343Δ, respectively. (d,f,h) RT–qPCR experiments measured nc-1343, nc-tgp1 and tgp1+ transcript levels in wild-type, rrp6Δ, mmi1Δ and 1343Δ, respectively. Error bars represent s.e.m. resulting from at least three independent replicates.