Figure 3. nc-tgp1, not nc-1343, represses tgp1⁺ to confer drug tolerance.

(a) Schematic diagram indicating strategic manipulations of lncRNAs upstream of tgp1⁺ including 1343Δ, shorter deletions of ncRNA.1343 (AΔ and BΔ) and ura4+ integration within the nc-tgp1 lncRNA locus (nc-tgp1:ura4⁺) in wild-type background. (b) Serial dilutions of wild-type, 1343Δ, AΔ, BΔ and nc-tgp1:ura4⁺ were spotted on non-selective YES medium or in the presence of TBZ (20 μg ml⁻¹), HU (10 mM) or caffeine (15 mM), respectively. (c) RT–qPCR experiments measured tgp1⁺, nc-tgp1 and nc-1343 transcript levels in wild-type, 1343Δ, AΔ, BΔ and nc-tgp1:ura4⁺ cells. Error bars represent s.e.m. resulting from three independent replicates.