Figure 5. tgp1⁺ is not regulated by RNAi/heterochromatin.

(a) H3K9me2 ChIP–qPCR experiments performed in the presence or absence of phosphate. clr4Δ was used as a negative control. The euchromatic actin gene (act1⁺) and centromeric dg repeats (dg) are positive and negative controls for heterochromatin. pho1⁺ is a phosphate-regulated gene repressed by nc-pho1, a lncRNA target of Mmi1. sme2⁺ is another lncRNA target of Mmi1. H3K9me2 to bulk H3 ratio has not been presented due to background methyl H3K9 levels detected at these loci. (b) RT–qPCR experiments measured tgp1⁺, nc-tgp1 and nc-1343 transcript levels in wild-type cells and cells lacking factors involved in heterochromatin formation and stability, respectively. Error bars represent s.e.m. resulting from at least three independent replicates.