Figure 6. nc-tgp1 transcription prevents stable Pho7 binding and increases nucleosome density upstream of tgp1⁺.

(a) GFP ChIP–qPCR experiments were performed in the presence or absence of phosphate in cells with C-terminally GFP-tagged Pho7. An untagged strain was used as a negative control. Primer pair #3 was used to detect Pho7 binding at the tgp1⁺ promoter. (b) Nucleosome density was measured by histone H3 ChIP–qPCR experiments in wild-type cells grown in the presence or absence of phosphate. Error bars represent s.e.m. resulting from three independent replicates.