Figure 7. nmt1 controlled nc-tgp1 alters drug tolerance in response to thiamine.

(a) Schematic diagram of nc-tgp1 under the control of the strong, thiamine-repressible nmt1 promoter. (b) RT–qPCR experiments measured tgp1⁺ and nc-tgp1 levels in response to thiamine and phosphate availability using nmt1-nc-tgp1 cells. (c) Serial dilutions of wild-type, 1343Δ and nmt1-nc-tgp1 cells were spotted on non-selective PMG medium or in the presence of TBZ, HU or caffeine, respectively, with or without thiamine as indicated. (d) H3 ChIP–qPCR experiments in nmt1-nc-tgp1 cells grown in the presence or absence of thiamine. Error bars represent s.e.m. resulting from three independent replicates.