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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: Nat Neurosci. 2014 Nov 10;17(12):1744–1750. doi: 10.1038/nn.3861

Figure 4. Inhibition of PBN LepRb neurons blunts the hyperglycemic response to glucoprivation.

Figure 4

(a) Bilateral injection of the cre-inducible AAV-hM4Di into the PBN of male Leprcre mice induces the expression of the inhibitory DREADD hM4Di in PBN LepRb neurons. Animals were injected with Vehicle or CNO (Injx: 0.3 mg/kg, IP) and then with 2DG (250 mg/kg, IP) and blood glucose was monitored for 240 minutes. (b) Blood glucose was monitored at the indicated times; *p=0.005 (15min), 0.017 (30min), 0.021 (60min), <0.001 (90min), <0.001 (120min), 0.004 (180min), <0.001 (240min) {F(9,38)=1.91} N=20 (veh) and 20 (cno) animals. (c) Area under the curve (AUC) for the data graphed in (b); p<0.001 {t(38)=4.22} N=20 (veh) and 20 (cno) animals. all data are plotted as mean +/− SEM.The data in b were analyzed by two way repeated measures ANOVA with Fisher LSD post hoc test; c was analyzed by two tailed t-test. Data in a is plotted as mean +/− SEM and as Q1, Q2, and Q3 with whiskers at the 10th and 90th percentiles in b.