Extended Data Figure 1.

Comparative analysis of metabolites significantly accumulated in ischaemic conditions. a, Various murine tissues exposed to sufficient periods of ischaemia to prime for reperfusion ROS production were subjected to targeted LC-MS metabolomics analysis and comparison of metabolites that accumulated significantly when compared to normoxic levels. Following this, metabolites were scored according to the prevalence of their accumulation across five ischaemic tissue conditions. B = brain, H = whole heart ischaemia ex vivo, HL = LAD ischaemia in vivo, K = kidney, L = liver. b, Determination of linearity of the relationship between LC-MS metabolite peak intensity and concentration for CAC and related metabolites. c, Quality control determination of coefficient of variation for LC-MS quantification of CAC and related metabolites.